产品名称	VCaP人前列腺癌细胞
货号	ZQ0353
产品介绍	1997从一位不受激素影响的前列腺癌患者脊椎转移灶中建立了这株细胞。先在小鼠中进行异种移植传代，随后进行体外培养。体内及体外都对雄性激素敏感。需用Gibco TrypLE Express进行消化。预定此细胞需至少提前两周。 特别注意： （1）VCaP细胞长得较慢，传代后细胞需要 48 小时贴壁，细胞需在48小时后换液操作。刚复苏后长到50%融合度需要1-2周，并且有时会出现漂浮的细胞团块和细胞碎片，这些属于正常现象。建议按照我们推荐的接种密度传代和维持培养。 （2）如果观察有小细胞团边缘透亮显示细胞没有完全贴 壁。正常细胞形态呈块状聚团生长。Vcap细胞贴壁后呈现许多小的紧密连接的细胞团块以及单细胞。如果传代或换液时有许多漂浮的细胞，请收集并离心，这些细胞可以继续培养。
种属	人
性别/年龄	男/59岁
组织	前列腺；来源于转移部位:脊椎转移
疾病	癌症
细胞类型	上皮细胞
形态学	上皮的
生长方式	贴壁
倍增时间	大约51~220小时
培养基和添加剂	DMEM高糖（品牌：中乔新舟 货号：ZQ-100）+10%胎牛血清（中乔新舟 货号：ZQ0500）+1%P/S（中乔新舟  货号：CSP006）
推荐完全培养基货号	ZM0353
生物安全等级	BSL-2
STR位点信息	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 9 D5S818: 12 D7S820: 9,12 TH01: 9.3 TPOX: 8,11 vWA: 18,19
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达
基因表达
保藏机构	ATCC; CRL-2876 ECACC; 06020201
供应限制	仅供科研使用

货号	ZQ0353
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

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