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NCI-H1395人肺腺癌细胞 (STR鉴定)
英文名:NCI-H1395
货号:ZQ0393
价格:¥1400.00
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NCI-H1395人肺腺癌细胞 (STR鉴定)

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NCI-H1395人肺腺癌细胞专用培养基

¥350.00 ¥480.00

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细胞套餐惊爆价

¥1750 ¥1880.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

NCI-H1395人肺腺癌细胞

货号

ZQ0393

产品介绍

NCI-H1395 [H1395] 是一种表现出上皮样形态的细胞系,分离自一名患有腺癌的 55 岁白人女性,该患者有大量吸烟史(按 15 包/年定量)。该细胞系建立于 1986 年,是肺癌研究中的重要生物学模型,特别是用于研究肺腺癌的病理生理学和治疗反应。该细胞系因其源自原发性肿瘤而闻名,为直接来自患者的肿瘤细胞的特征和行为提供了宝贵的见解。

ATCC CRL-5957是一株来源于同一患者的类淋巴母细胞。

注意事项:
(1)NCI-H1395细胞消化后培养24h后还有大量圆细胞,48小时后细胞才会贴壁,细胞48小时后换液操作,
(2)在细胞换液过程中一定不要对细胞面加液,否则细胞会脱落。由于细胞贴壁不牢特点,建议使用Corning CellBIND(3289)培养瓶细胞更容易贴壁。

种属

性别/年龄

女/55岁

组织

疾病

2期,腺癌

细胞类型

肿瘤细胞

形态学

上皮的

生长方式

贴壁

倍增时间

大约50.5小时

培养基和添加剂

RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%FBS(品牌:中乔新舟 货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006)+1% Sodium Pyruvate 100 mM Solution(中乔新舟  货号:CSP003)+1%L-alanyl-L-glutamine(中乔新舟   货号:CSP004

推荐完全培养基货号

ZM0393

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

CSF1PO: 12

D13S317: 10,14

D16S539: 11,13

D5S818: 12

D7S820: 8

TH01: 6,9.3

TPOX: 8

vWA: 14,17

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC; CRL-5868

供应限制

仅供科研使用

货号

ZQ0393

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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