产品名称 |
RD人恶性胚胎横纹肌瘤细胞 |
货号 |
ZQ0401 |
产品介绍 |
目前显示,这株细胞如果跟TE 671 (ATCC HTB-139)不一样,至少也是它的亲本。横纹肌肉瘤(RD)细胞是从肌肉组织中分离出的大型多核纺锤形细胞。使用该细胞系进行研究和病毒检测。 |
种属 |
人 |
性别/年龄 |
女/7周 |
组织 |
肌肉 |
疾病 |
横纹肌肉瘤 |
细胞类型 |
肿瘤细胞 |
形态学 |
梭形细胞和大型多核细胞 |
生长方式 |
贴壁 |
倍增时间 |
大约23小时 |
培养基和添加剂 |
DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 10,11 D5S818: 11 D7S820: 8,12 THO1: 9.3 TPOX: 9 vWA: 18 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CCL-136 ATCC; CRL-7713 ATCC; CRL-7731 ATCC; HTB-97 ECACC; 85111502 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0401 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=4241949; DOI=10.1002/1097-0142(196909)24:3<520::AID-CNCR2820240313>3.0.CO;2-M
McAllister R.M., Melnyk J., Finkelstein J.Z., Adams E.C. Jr., Gardner M.B.
Cultivation in vitro of cells derived from a human rhabdomyosarcoma.
Cancer 24:520-526(1969)
PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)
PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)
PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x
Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.
Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.
Tissue Antigens 11:279-286(1978)
PubMed=7309287; DOI=10.1002/ijc.2910280403
Houghton J.A., Houghton P.J., Brodeur G.M., Green A.A.
Development of resistance to vincristine in a childhood rhabdomyosarcoma growing in immune-deprived mice.
Int. J. Cancer 28:409-415(1981)
PubMed=6220172
Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)
PubMed=3158613; DOI=10.1002/ijc.2910350513
Chardin P., Yeramian P.D., Madaule P., Tavitian A.
N-ras gene activation in the RD human rhabdomyosarcoma cell line.
Int. J. Cancer 35:647-652(1985)
PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)
PubMed=2216456
Stratton M.R., Moss S., Warren W., Patterson H., Clark J., Fisher C., Fletcher C.D.M., Ball A., Thomas M., Gusterson B.A., Cooper C.S.
Mutation of the p53 gene in human soft tissue sarcomas: association with abnormalities of the RB1 gene.
Oncogene 5:1297-1301(1990)
PubMed=2302710
Ozkaynak M.F., Nolta J., Parkman R.
In vitro purging of human rhabdomyosarcoma cells using 4-hydroperoxycyclophosphamide.
Cancer Res. 50:1455-1458(1990)
PubMed=1917385
Lollini P.-L., De Giovanni C., Landuzzi L., Nicoletti G., Scotlandi K., Nanni P.
Reduced metastatic ability of in vitro differentiated human rhabdomyosarcoma cells.
Invasion Metastasis 11:116-124(1991)
PubMed=8275086; DOI=10.1038/ng1193-230
Galili N., Davis R.J., Fredericks W.J., Mukhopadhyay S., Rauscher F.J. III, Emanuel B.S., Rovera G., Barr F.G.
Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma.
Nat. Genet. 5:230-235(1993)
PubMed=8383879; DOI=10.1126/science.8383879
Tapscott S.J., Thayer M.J., Weintraub H.M.
Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis.
Science 259:1450-1453(1993)
PubMed=10899454; DOI=10.1016/S0960-8966(00)00096-1
Vergani L., Prescott A.R., Holt I.J.
Rhabdomyosarcoma rho(0) cells: isolation and characterization of a mitochondrial DNA depleted cell line with 'muscle-like' properties.
Neuromuscul. Disord. 10:454-459(2000)
PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)
PubMed=16518851; DOI=10.1002/gcc.20322
Chen Y.-Y., Takita J., Hiwatari M., Igarashi T., Hanada R., Kikuchi A., Hongo T., Taki T., Ogasawara M., Shimada A., Hayashi Y.
Mutations of the PTPN11 and RAS genes in rhabdomyosarcoma and pediatric hematological malignancies.
Genes Chromosomes Cancer 45:583-591(2006)
PubMed=17471488; DOI=10.1002/path.2170
Lae M., Ahn E.H., Mercado-Celis G.E., Chuai S., Edgar M., Pawel B.R., Olshen A.B., Barr F.G., Ladanyi M.
Global gene expression profiling of PAX-FKHR fusion-positive alveolar and PAX-FKHR fusion-negative embryonal rhabdomyosarcomas.
J. Pathol. 212:143-151(2007)
PubMed=18082704; DOI=10.1016/j.jpedsurg.2007.08.026
Komuro H., Saihara R., Shinya M., Takita J., Kaneko S., Kaneko M., Hayashi Y.
Identification of side population cells (stem-like cell population) in pediatric solid tumor cell lines.
J. Pediatr. Surg. 42:2040-2045(2007)
PubMed=19235922; DOI=10.1002/gcc.20655
Missiaglia E., Selfe J., Hamdi M., Williamson D., Schaaf G., Fang C., Koster J., Summersgill B.M., Messahel B., Versteeg R., Pritchard-Jones K., Kool M., Shipley J.M.
Genomic imbalances in rhabdomyosarcoma cell lines affect expression of genes frequently altered in primary tumors: an approach to identify candidate genes involved in tumor development.
Genes Chromosomes Cancer 48:455-467(2009)
PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)
PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)
PubMed=20922763; DOI=10.1002/pbc.22801
Kang M.H., Smith M.A., Morton C.L., Keshelava N., Houghton P.J., Reynolds C.P.
National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.
Pediatr. Blood Cancer 56:239-249(2011)
PubMed=21948088; DOI=10.1158/1078-0432.CCR-11-1004
Belyea B.C., Naini S., Bentley R.C., Linardic C.M.
Inhibition of the Notch-Hey1 axis blocks embryonal rhabdomyosarcoma tumorigenesis.
Clin. Cancer Res. 17:7324-7336(2011)
PubMed=22142829; DOI=10.1158/1078-0432.CCR-11-2056
Shukla N., Ameur N., Yilmaz I., Nafa K., Lau C.-Y., Marchetti A., Borsu L., Barr F.G., Ladanyi M.
Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways.
Clin. Cancer Res. 18:748-757(2012)
PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)
PubMed=22541669; DOI=10.1186/2044-5040-2-7
MacQuarrie K.L., Yao Z.-Z., Young J.M., Cao Y., Tapscott S.J.
miR-206 integrates multiple components of differentiation pathways to control the transition from growth to differentiation in rhabdomyosarcoma cells.
Skelet. Muscle 2:7.1-7.14(2012)
PubMed=23578105; DOI=10.1111/cas.12173
Nishimura R., Takita J., Sato-Otsubo A., Kato M., Koh K., Hanada R., Tanaka Y., Kato K., Maeda D., Fukayama M., Sanada M., Hayashi Y., Ogawa S.
Characterization of genetic lesions in rhabdomyosarcoma using a high-density single nucleotide polymorphism array.
Cancer Sci. 104:856-864(2013)
产品名称 | 价格 | 指令 |
支原体检测试剂盒 | ¥800.00 | 购物车 》 |
支原体清除试剂盒 | ¥600.00 | 购物车 》 |
国产转染试剂 | ¥800.00 | 购物车 》 |
慢病毒介导基因沉默或过表达 | ¥询价 | 购物车 》 |
荧光素酶标记 | ¥询价 | 购物车 》 |
绿/红色荧光蛋白标记 | ¥询价 | 购物车 》 |
DMEM(高糖)基础培养基 | ¥56.00 | 购物车 》 |
DMEM(高糖)完全培养基 | ¥350.00 | 购物车 》 |
优级胎牛血清 | ¥2580(开学促销) | 购物车 》 |
支原体抑制剂 10 mg/ml; 1 ml(定制) | ¥1808.00 | 购物车 》 |
人源细胞STR鉴定服务 | ¥900.00 | 购物车 》 |