人肺微血管内皮细胞说明：
肺微血管内皮细胞构成半选择性屏障，该屏障对于肺气体交换，调节液体和可溶物在血液与肺间质之间的流动具有重要意义。它还具有代谢功能，可以执行一定的非呼吸功能。在肺损伤中，PMECs是活性氧类的重要靶细胞之一。在肺炎的发生过程中，神经体液介质和氧化剂作用于内皮细胞，使得细胞间隙渗透性增加，蛋白质由血液进入间质。细胞间隙渗透性的增加导致低氧血症，出现成人呼吸窘迫综合征和非心源性肺水肿。
人肺微血管内皮细胞(HPMEC)提取于人肺组织，细胞提取纯化之后，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到10倍增。
配套培养基：内皮细胞专用全能培养基ECM（NO.1001）
产品使用说明：仅供科研研究使用
1.) Chen, Q.H., Liu, A.R., Qiu, H.B. & Yang, Y. (2015) Interaction between mesenchymal stem cells and endothelial cells restores endothelial permeability via paracrine hepatocyte growth factor in vitro Stem Cell Res Ther. 6
2.) Lamichhane, S.P., Arya, N., Ojha, N., Kohler, E. & Shastri, V.P. (2015) Glycosaminoglycan-functionalized poly-lactide-co-glycolide nanoparticles: synthesis, characterization, cytocompatibility, and cellular uptake Int J Nanomedicine. 10
3.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
4.) Menden, H., Welak, S., Cossette, S., Ramchandran, R. & Sampath, V. (2015) Lipopolysaccharide (LPS)-mediated Angiopoietin-2-dependent Autocrine Angiogenesis Is Regulated by NADPH Oxidase 2 (Nox2) in Human Pulmonary Microvascular Endothelial Cells* J Biol Chem. 290
5.) Nicol�, E., Syga, M.I., Bosetti, M. & Shastri, V.P. (2015) Enhanced Gene Silencing through Human Serum Albumin-Mediated Delivery of Polyethylenimine-siRNA Polyplexes PLoS One. 10
6.) Yang, Y., Chen, Q., Liu, A., Xu, X., Han, J. & Qiu, H. (2015) Synergism of MSC-secreted HGF and VEGF in stabilising endothelial barrier function upon lipopolysaccharide stimulation via the Rac1 pathway Stem Cell Res Ther. 6
7.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) 'Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains.'
8.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) "Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains." Infect immune. 82: 949-59.
9.) Claessens A, Adams Y, Ghumra A, Lindergard G, Buchan CC, Andisi C, Bull PC, Mok S, Gupta AP, Wang CW, Turner L, Arman M, Raza A, Bozdech Z, Rowe JA. (2012) "A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells." Proc Natl Acad Sci USA.109: E1772-81.
10.) Kang CY, Zou L, Yuan M, Wang Y, Li TZ, Zhang Y, Wang JF, Li Y, Deng XW, Liu CT. (2011) "Impact of simulated microgravity on microvascular endothelial cell apoptosis."Eur J Appl Physiol. 111: 2131-8.
11.) Reutershan J, Saprito MS, Wu D, R?ckle T, Ley K. (2010) "Phosphoinositide 3-kinase gamma required for lipopolysaccharide-induced transepithelial neutrophil trafficking in the lung." Eur Respir J. 35: 1137-47.
12.) Rydkina E, Turpin LC, Sahni SK. (2010) "Rickettsia rickettsii infection of human macrovascular and microvascular endothelial cells reveals activation of both common and cell type-specific host response mechanisms." Infect Immun. 78: 2599-606.
13.) Shahani T, Lavend'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. (2010) "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool." Blood. 115: 4902-9.
14.) Wright CJ, Agboke F, Chen F, LA P, Yang G, Dennery PA. (2010) "Nitric Oxide Inhibits Hyperoxia-induced NF-kappaB Activation in Neonatal Pulmonary Microvascular Endothelial Cells." Pediatr Res. 68: 484-9.
15.) Oca?a-Macchi M, Bel M, Guzylack-Piriou L, Ruggli N, Liniger M, McCullough KC, Sakoda Y, Isoda N, Matrosovich M, Summerfield A. (2009) "Hemagglutinin-dependent tropism of H5N1 avian influenza virus for human endothelial cells." J Virol. 83: 12947-55.
16.) Wolters PJ, Wray C, Sutherland RE, Kim SS, Koff J, Mao Y, Frank JA. (2009) "Neutrophil-derived IL-6 limits alveolar barrier disruption in experimental ventilator-induced lung injury." J Immunol. 182: 8056-62.
17.) Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J. (2008) "Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis." Blood. 112: 340-9.
18.) Kiss J, Yegutkin GG, Koskinen K, Savunen T, Jalkanen S, Salmi M. (2007) "IFN-beta protects from vascular leakage via up-regulation of CD73." Eur J Immunol. 37: 3334-8.
19.) Moldobaeva A, Welsh-Servinsky LE, Shimoda LA, Stephens RS, Verin AD, Tuder RM, Pearse DB. (2006) "Role of protein kinase G in barrier-protective effects of cGMP in human pulmonary artery endothelial cells." Am J Physiol Lung Cell Mol Physiol. 290: L919-30.
20.) Kanmogne GD, Primeaux C, Grammas P. (2005) "Induction of apoptosis and endothelin-1 secretion in primary human lung endothelial cells by HIV-1 gp120 proteins."Biochem Biophys Res Commun. 333: 1107-15.
21.)Modeling tumor phenotypes in vitro with three-dimensional bioprinting,Cell Rep. 2019 Jan 15; 26(3): 608–623.e6.doi: 10.1016/j.celrep.2018.12.090
22.）An oncolytic herpes virus expressing E-cadherin resists NK cell clearance and improves viral spread and glioblastoma virotherapy,Nat Biotechnol. 2018 Nov 26 : 10.1038/nbt.4302.Published online 2018 Nov 26. doi: 10.1038/nbt.4302
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