人Notch信号通路定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Notch Signaling Pathway qPCR array kit (GQH-NCH) is designed to facilitate gene expression profiling of 88 key genes involved in the human Notch molecular pathway with an emphasis on Notch1 signal transduction. Brief examples of how included genes may be grouped according to their roles are shown below:
Canonical ligands: JAG1, JAG2, DLL1, DLL3DLL4
Noncanonical ligands: WNT1, DNER, CNTN1, NOV, SHH
Receptors: NOTCH1, NOTCH2, NOTCH3, NOTCH4
Transcriptional repressors: HEY1, HEY2, ESR1, HES1, HES7
Proteolysis: ADAM17, PSEN1, FURIN, POFUT1, PSENEN
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Notch Signaling Pathway qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ACBD3 |
CDKN1A |
DLL1 |
DVL2 |
GSK3B |
IL2RA |
MAML3 |
NCOR2 |
NRARP |
PSENEN |
SMAD2 |
β-actin |
|
B |
ADAM10 |
CIR1 |
DLL3 |
EGFL7 |
HES1 |
JAG1 |
MEF2C |
NEURL1 |
NUMB |
PTF1A |
SMO |
GAPDH |
|
C |
ADAM17 |
CLTA |
DLL4 |
ESR1 |
HES5 |
JAG2 |
MFAP2 |
NFKB2 |
NUMBL |
RBPJ |
SNW1 |
LDHA |
|
D |
APH1A |
CNTN1 |
DNAAF3 |
ESR2 |
HES7 |
JUN |
MFAP5 |
NOTCH1 |
PCSK5 |
RFNG |
SPEN |
NONO |
|
E |
AXIN1 |
CNTN6 |
DNER |
FBXW7 |
HEY1 |
LFNG |
MFNG |
NOTCH2 |
POFUT1 |
RITA1 |
TBP |
PPIH |
|
F |
CCND1 |
CTNNB1 |
DNM1 |
FHL1 |
HEY2 |
MAGI2 |
MIB1 |
NOTCH3 |
POGLUT1 |
RUNX1 |
TCF7L2 |
GDC |
|
G |
CDK2 |
DLK1 |
DTX1 |
FURIN |
HEYL |
MAML1 |
MIB2 |
NOTCH4 |
PSEN1 |
SHH |
THBS2 |
PPC |
|
H |
CDK8 |
DLK2 |
DTX4 |
GATA3 |
HIF1A |
MAML2 |
MLLT4 |
NOV |
PSEN2 |
SIRT1 |
WNT1 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK025 |
|
产地 |
美国 |
|
缩写 |
GQH-NCH |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 |
| 1520 | 人神经元 | 1 x 10^6 cells/vial | ￥14220.00 | 放入购物车 》 |
| 1650 | HiPSC人源性神经干细胞 | 1 x 10^6 cells/vial | ￥13500.00 | 放入购物车 》 |
| 3830 | 人胰腺星状细胞 | 5 x 10^5 cells/vial | ￥14364.00 | 放入购物车 》 |
| 4610 | 人股骨成骨细胞 | 5x10^5cells/vial | ￥14382.00 | 放入购物车 》 |
| 6020 | 人冠状动脉内皮细胞 | 5 x 10^5 cells/vial | ￥14040.00 | 放入购物车 》 |
| 1001 | 内皮细胞培养基 | 500ML | ￥2224.00 | 放入购物车 》 |
| 4601 | 成骨细胞培养基 | 500ML | ￥2192.00 | 放入购物车 》 |
| 5301 | 星形细胞培养基 | 500ML | ￥2256.00 | 放入购物车 》 |
| 7501 | 间充质干细胞培养基 | 500ML | ￥2320.00 | 放入购物车 》 |
| 5931 | 多功能干细胞神经诱导培养基 | 500ML，100ML | ￥2688.00-7520.00 | 放入购物车 》 |
| 7520 | 人间充质干细胞--肝脏 | 5 x 10^5 cells/vial | ￥14616.00 | 放入购物车 》 |
| 1524 | 人神经元细胞cDNA | 20 reactions | ￥6552.00 | 放入购物车 》 |
| 3834 | 人胰腺星状细胞cDNA | 20 reactions | ￥7087.00 | 放入购物车 》 |
| 4614 | 人股骨成骨细胞cDNA | 20 reactions | ￥6552.00 | 放入购物车 》 |
| 6024 | 人冠状动脉内皮细胞cDNA | 20 reactions | ￥7087.00 | 放入购物车 》 |
| 7524 | 人间充质干细胞-肝cDNA | 20 reactions | ￥7087.00 | 放入购物车 》 |
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