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NCI-H522人非小细胞肺癌腺癌细胞(STR鉴定)

英文名:NCI-H522
货号:ZQ0901
价格:¥1800.00
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NCI-H522人非小细胞肺癌腺癌细胞(STR鉴定)

¥1800.00
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NCI-H522人非小细胞肺癌腺癌细胞专用培养基

¥350.00 ¥450.00

配套完培,省时省力,单买细胞无优惠

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¥2150 ¥2650.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

NCI-H522人非小细胞肺癌腺癌细胞

货号

ZQ0901

产品介绍

NCI-H522是一种人非小细胞肺癌腺癌细胞系,最初由A.F. Gazdar、H.K. Oie、J.D. Minna及其同事从一名58岁白色男性吸烟肺癌患者治疗前获得的。这种细胞系具有亚三倍体的染色体特征,模态染色体数出现在68%的计数细胞中,并且所有细胞共有11条标记染色体,其中两条是配对的。这些细胞携带TP53基因密码子191的突变,并且不能合成肽神经调节蛋白B (NMB)或胃泌素释放肽 (GRP)。此外,细胞对角蛋白和波形蛋白染色呈阳性,但对神经丝三联体蛋白呈阴性。

NCI-H522细胞系因其特性被广泛应用于非小细胞肺癌的研究,包括病理机制、药物筛选和基因功能研究。

种属

性别/年龄

男/58岁

组织

疾病

腺癌;非小细胞肺癌;第二阶段

细胞类型

肿瘤细胞 

形态学

上皮的

生长方式

贴壁

倍增时间

大约20~48.1小时

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟  货号:ZQ0500)+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0901

生物安全等级

BSL-1

STR位点信息

Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 12
D5S818: 11
D7S820: 9,10
TH01: 7,9.3
TPOX: 9,10
vWA: 17
D3S1358: 16
D21S11: 28,31.2
D18S51: 14,18
Penta_E: 11
Penta_D: 11
D8S1179: 12,13
FGA: 24
D19S433: 16
D2S1338: 17,20

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC; CRL-5810 KCLB; 90522

供应限制

仅供科研使用

货号

ZQ0901

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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