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人肾癌定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Renal Cell Carcinoma qPCR Array Kit (GQH-RCC) is designed to facilitate gene expression profiling of key genes involved in (i) renal cell carcinoma (RCC) biology and carcinogenesis, including subtypes such as conventional (clear cell), papillary, chromophobe, and collecting duct RCCs; and (ii) the regulation pathway of beta-cell development. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Renal Cell Carcinoma qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AKT1 |
CAV1 |
CUL2 |
EPAS1 |
GAB1 |
KIT |
MOK |
PAK6 |
PIK3R1 |
RAP1B |
TCEB1 |
β-actin |
|
B |
APEH |
CDC42 |
DIRC2 |
EPCAM |
GAL3ST1 |
KRAS |
MUC1 |
PAK7 |
PIK3R5 |
RAPGEF1 |
TCEB2 |
GAPDH |
|
C |
ARAF |
CDH16 |
DNAJB8 |
ETS1 |
GRB2 |
KRT7 |
NRAS |
PAX8 |
PRCC |
RBX1 |
TFE3 |
LDHA |
|
D |
ARNT |
CLDN8 |
EGFR |
FAM107A |
HGF |
MAP2K2 |
OGG1 |
PBRM1 |
PSMB9 |
RNF139 |
TGFA |
NONO |
|
E |
ARNT2 |
CREBBP |
EGLN1 |
FH |
HNF1A |
MAPK1 |
PAK1 |
PCNA |
PTPN11 |
S100A1 |
TGFB1 |
PPIH |
|
F |
ASPSCR1 |
CRK |
EGLN2 |
FHIT |
JUN |
MAPK3 |
PAK2 |
PDGFB |
RAC1 |
SLC2A1 |
VEGFA |
GDC |
|
G |
BRAF |
CRKL |
EGLN3 |
FIGF |
KANK1 |
MET |
PAK3 |
PDGFRB |
RAF1 |
SOS1 |
VHL |
PPC |
|
H |
CA9 |
CSF2 |
EP300 |
FLCN |
KDR |
MME |
PAK4 |
PIK3CA |
RAP1A |
SOS2 |
VIM |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK016 |
|
产地 |
美国 |
|
缩写 |
GQH-RCC |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
|
参考文献 |
[1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
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