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人肾癌定量PCR芯片试剂盒
英文名:GeneQuery™ Human Renal Cell Carcinoma qPCR Array Kit
货号:GK016
价格:¥4320.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

人肾癌定量PCR芯片试剂盒描述:


       ScienCell's GeneQuery™ Human Renal Cell Carcinoma qPCR Array Kit (GQH-RCC) is designed to facilitate gene expression profiling of key genes involved in (i) renal cell carcinoma (RCC) biology and carcinogenesis, including subtypes such as conventional (clear cell), papillary, chromophobe, and collecting duct RCCs; and (ii) the regulation pathway of beta-cell development. 88 genes are selected in this kit based on database and literature research.      


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 


       GeneQuery™ Human Renal Cell Carcinoma qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

CAV1

CUL2

EPAS1

GAB1

KIT

MOK

PAK6

PIK3R1

RAP1B

TCEB1

β-actin

B

APEH

CDC42

DIRC2

EPCAM

GAL3ST1

KRAS

MUC1

PAK7

PIK3R5

RAPGEF1

TCEB2

GAPDH

C

ARAF

CDH16

DNAJB8

ETS1

GRB2

KRT7

NRAS

PAX8

PRCC

RBX1

TFE3

LDHA

D

ARNT

CLDN8

EGFR

FAM107A

HGF

MAP2K2

OGG1

PBRM1

PSMB9

RNF139

TGFA

NONO

E

ARNT2

CREBBP

EGLN1

FH

HNF1A

MAPK1

PAK1

PCNA

PTPN11

S100A1

TGFB1

PPIH

F

ASPSCR1

CRK

EGLN2

FHIT

JUN

MAPK3

PAK2

PDGFB

RAC1

SLC2A1

VEGFA

GDC

G

BRAF

CRKL

EGLN3

FIGF

KANK1

MET

PAK3

PDGFRB

RAF1

SOS1

VHL

PPC

H

CA9

CSF2

EP300

FLCN

KDR

MME

PAK4

PIK3CA

RAP1A

SOS2

VIM

NTC

 

* gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK016

产地

美国

缩写

GQH-RCC

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

参考文献

[1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12.


       [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12.
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