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人转录误调节在实体肿瘤中的定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Transcriptional Misregulation in Solid Tumors qPCR Array Kit (GQH-TMS) is designed to facilitate gene expression profiling of 88 key genes involved in six types of solid tumor development (prostate cancer, kidney cancer, thyroid cancer, neuroblastoma, carcinoid, and sarcomas). Brief examples of how included genes may be grouped according to function and tumor types are shown below:
Cell cycle regulation: CDK9, CDKN1A, CDKN1B
Apoptosis and proliferation: TP53, RXRs, PPARG, MDM2
Cellular invasion: MMP9, SPINT1, IL1R2, PLAU, PLAT, ZEB1
Cell survival: BMI1, MYC, IGF1, MLF1
Mutated transcription factors identified in
prostate cancer: ERG, ETV1, ETV4, ELK4
kidney cancer: TFE3
thyroid cancer: PAX8
neuroblastoma: MYCN
sarcomas: ATF1, FEV, WTI, DDIT3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Transcriptional Misregulation in Solid Tumors qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AFF1 |
CCNT2 |
DDX5 |
FUS |
HIST1H3D |
HIST2H3D |
MAX |
MYC |
PAX8 |
SLC45A3 |
TFE3 |
β-actin |
|
B |
ARNT2 |
CDK9 |
ELK4 |
GOLPH3 |
HIST1H3E |
HIST3H3 |
MDM2 |
MYCN |
PLAT |
SMAD1 |
TGFBR2 |
GAPDH |
|
C |
ASPSCR1 |
CDKN1A |
ERG |
GOLPH3L |
HIST1H3F |
IGF1 |
MEN1 |
NFKB1 |
PLAU |
SP1 |
TMPRSS2 |
LDHA |
|
D |
ATF1 |
CDKN1B |
ETV1 |
H3F3B |
HIST1H3G |
IGF1R |
MET |
NFKBIZ |
PPARG |
SPINT1 |
TP53 |
NONO |
|
E |
ATM |
CEBPB |
ETV4 |
H3F3C |
HIST1H3H |
IL1R2 |
MLF1 |
NGFR |
PRCC |
SS18 |
TSPAN7 |
PPIH |
|
F |
BAIAP3 |
COMMD3 |
EWSR1 |
HIST1H3A |
HIST1H3I |
IL2RB |
MLLT1 |
NTRK1 |
RXRA |
SSX2 |
WT1 |
GDC |
|
G |
BMI1 |
CXCL8 |
FEV |
HIST1H3B |
HIST1H3J |
IL6 |
MLLT3 |
NUPR1 |
RXRB |
SSX2B |
ZBTB17 |
PPC |
|
H |
CCNT1 |
DDIT3 |
FOXO1 |
HIST1H3C |
HIST2H3A |
KMT2A |
MMP9 |
PAX7 |
RXRG |
TAF15 |
ZEB1 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK031 |
|
产地 |
美国 |
|
缩写 |
GQH-TMS |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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