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人II型糖尿病(晚发性、胰岛素抗性)定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Diabetes, Type II (Late-onset, Insulin Resistance) qPCR Array Kit (GQH-DB2) is designed to facilitate gene expression profiling of 88 key genes involved in the development and progression of late-onset diabetes mellitus. Type 2 diabetes mellitus is characterized by abnormally high blood sugar levels resulting from a failure to utilize insulin properly. Brief examples of how included genes may be grouped according to function are shown below:
Blood glucose regulation: INS, INSR, SLC2A2, SLC30A8, ABCC8
Fat regulation: ADIPOQ, LPL, PON1, LEP, RETN
Cholesterol regulation: APOA1, APOB, LPA, CETP
Metabolism: UCP2, GCG, PCK2, GYS1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Diabetes, Type II (Late-onset, Insulin resistance) qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABCC8 |
AKT2 |
CETP |
GCK |
HNF1A |
IGFBP3 |
LEP |
NEUROD1 |
PON1 |
SELE |
TCF4 |
β-actin |
|
B |
ACE |
ALB |
COG2 |
GFPT1 |
HNF1B |
IL6 |
LEPR |
NOS3 |
PPARA |
SERPINA12 |
TCF7L2 |
GAPDH |
|
C |
ADIPOQ |
ALKBH3 |
CRP |
GHRL |
HNF4A |
IMMP2L |
LIPC |
PAX4 |
PPARG |
SERPINE1 |
TNF |
LDHA |
|
D |
ADIPOR1 |
APOA1 |
DPP4 |
GPD2 |
HSD11B1 |
INS |
LIPH |
PCK2 |
PPARGC1A |
SLC2A1 |
UCP2 |
NONO |
|
E |
ADRB3 |
APOB |
EDN1 |
GYS1 |
IAPP |
INSR |
LPA |
PDX1 |
PPP1R3A |
SLC2A2 |
UCP3 |
PPIH |
|
F |
AGT |
APOC3 |
ENPP1 |
HES1 |
IGF1 |
IRS1 |
LPL |
PIK3R1 |
PTPN1 |
SLC2A4 |
VCAM1 |
GDC |
|
G |
AGTR1 |
CAPN10 |
GAD2 |
HK2 |
IGF2 |
IRS2 |
MAPK8IP1 |
PLAT |
RBP4 |
SLC30A8 |
VWF |
PPC |
|
H |
AKT1 |
CCL2 |
GCG |
HMGA1 |
IGFBP1 |
KCNJ11 |
MTNR1B |
PLTP |
RETN |
SOCS3 |
WFS1 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK051 |
|
产地 |
美国 |
|
缩写 |
GQH-DB2 |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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