人心脏微血管内皮细胞HCMEC

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人心脏微血管内皮细胞

英文名：Human Cardiac Microvascular Endothelial Cells
货号：6000
价格：￥14940.00

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心脏微血管内皮细胞描述：

内皮细胞通过分泌内皮源性血管收缩因子和内皮源性血管舒张因子，调节和降解血管活性肽以及内皮细胞表面的酶，达到进一步调节血管张力的功能。这些功能在不同的部位，不同器官的血管，以及大循环和微循环之间均存在着不同。人心脏微血管内皮细胞对于心肌细胞的功能同时具有生理和病理的效应。它通过分泌NO、内皮素-1、前列腺素、腺苷酸嘌呤、利钠肽以及其它的物质调节心肌细胞的功能。研究发现该效应在体内和体外培养都存在。CMEC也具有酶活性，特别是ACE/激肽酶活性，此酶活性能够调节心脏内局部血管紧张素II 和缓激肽的水平。

人心脏微血管内皮细胞(HCMEC)于人心脏组织分离提取，提取纯化之后立即冻存。属第一代冻存，每管含有细胞数>5×105cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到5倍增。

推荐使用培养液：内皮细胞专用培养液（ECM,NO.1001）

产品使用说明：仅供科研研究使用

货号	6000
产地	美国
缩写	HCMEC
规格	5 x 10^5 cells/vial
用途	科研
运输	干冰
保存	液氮

1.) Liu, K., Wang, X.J., Li, Y.N., Li, B., Qi, J.S., Zhang, J. & Wang, Y. (2016) Tongxinluo Reverses the Hypoxia-suppressed Claudin-9 in Cardiac Microvascular Endothelial CellsChinese Medical Journal. 129

2.) Hai-Yan Z, Yong-Hong G, Zhi-Yao W, Bing X, Ai-Ming W, Yan-Wei X,Bei L, Li-Xia L, Li-Xin C. (2013) "Astragalus Polysaccharide Suppresses the Expression of Adhesion Molecules through the Regulation of the p38 MAPK Signaling Pathway in Human Cardiac Microvascular Endothelial Cells after Ischemia-Reperfusion Injury." Evid Based Complement Alternat Med. 2013.

3.) Avril M, Tripathi AK, Brazier AJ, Andisi C, Janes JH, Soma VL, Sullivan DJ Jr, Bull PC, Stins MF, Smith JD. (2012) "A restricted subset of var genes mediates adherence of Plasmodium falciparum-infected erythrocytes to brain endothelial cells." Proc Natl Acad Sci USA. 109: E1782-90.

4.) Shahani T, Lavend'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. (2010) "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool." Blood. 115: 4902-9.

5.) Haudek SB, Gupta D, Dewald O, Schwartz RJ, Wei L, Trial J, Entman ML. (2009) "Rho kinase-1 mediates cardiac fibrosis by regulating fibroblast precursor cell differentiation." Cardiovasc Res. 83: 511-8.

6.) Chandrasekar B, Boylston WH, Venkatachalam K, Webster NJ, Prabhu SD, Valente AJ. (2008) "Adiponectin blocks interleukin-18-mediated endothelial cell death via APPL1-dependent AMP-activated protein kinase (AMPK) activation and IKK/NF-kappaB/PTEN suppression." J Biol Chem. 283: 24889-98.

7.) Zabalgoitia M, Colston JT, Reddy SV, Holt JW, Regan RF, Stec DE, Rimoldi JM, Valente AJ, Chandrasekar B. (2008) "Carbon monoxide donors or heme oxygenase-1 (HO-1) overexpression blocks interleukin-18-mediated NF-kappaB-PTEN-dependent human cardiac endothelial cell death." Free Radic Biol Med. 44: 284-98.

8.) Chandrasekar B, Vemula K, Surabhi RM, Li-Weber M, Owen-Schaub LB, Jensen LE, Mummidi S. (2004) "Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death." J Biol Chem. 279: 20221-33.

9.) Ray RB, Basu A, Steele R, Beyene A, McHowat J, Meyer K, Ghosh AK, Ray R. (2004) "Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells." Virology. 321: 181-8.

Question:1
In the Subculturing Protocol, No 5 is "Add 10ml of DPBS and then 2ml of T/E solution into flask". Is it put the T/E and DPBS together? is the T/E diluted?
Answers:
Posted by Karishma Shah on Friday, May 13, 2016
Dear customer,
T/E needs to be diluted by PBS. It cannot be directly added onto cells.

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