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NCI-H441人肺腺癌细胞 (STR鉴定)
英文名:NCI-H441
货号:ZQ0624
价格:¥1200.00
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NCI-H441人肺腺癌细胞 (STR鉴定)

¥1200.00
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NCI-H441人肺腺癌细胞专用培养基

¥350.00 ¥450.00

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¥1550 ¥3650.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

NCI-H441 人肺腺癌细胞

货号

ZQ0624

产品介绍

NCI-H441细胞系,也称为H441,是由A.F. Gazdar、M. Brower和D. Carney等人于1982年从一位患有肺乳头状腺癌的男性患者的胸水中建立的细胞系。这是一种经过良好特征化的上皮腺癌细胞系。这些细胞因其与肺上皮生物学的相关性,在生物研究中被广泛使用,是研究跨上皮运输和上皮屏障功能的体外模型。

NCI-H441细胞系是增进我们对肺部药物处置和肿瘤动力学理解的重要工具。在3D细胞培养模型中的使用,允许详细研究药物在肺部环境中的吸收、分布、代谢和排泄,密切模拟体内条件。

鉴于它们的起源和特性,NCI-H441细胞在专注于远端肺和相关疾病(包括肺腺癌)的研究中特别有价值,作为理解肺部疾病机制和评估潜在治疗干预的稳定和相关细胞模型。

NCI-H441细胞在3D细胞培养、高通量筛选和毒理学研究中发挥着重要作用,为细胞反应和治疗剂的有效性提供了宝贵的数据。人类细胞系H441的一个显著应用是作为转染宿主,用于表达肺表面活性蛋白(SP-B),利用萤火虫荧光素酶报告系统,这强调了它们在吸入生物药剂学和跨上皮运输研究中的作用。这种能力,加上它们对主要表面活性物质载脂蛋白(SP-A)的mRNA和蛋白的表达,突出了细胞系在研究肺功能和疾病中的重要性,特别是那些影响表面活性物质调节和合成的疾病

种属

性别/年龄

男/33岁

组织

疾病

乳头状腺癌

细胞类型

肿瘤细胞

形态学

上皮的

生长方式

贴壁

倍增时间

大约46.5~138小时

培养基和添加剂

RPMI-1640(品牌:中乔新舟 货号:ZQ-200+10%胎牛血清(中乔新舟 货号:ZQ0500+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0624

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 9

D16S539: 9,13

D5S818: 11,12

D7S820: 10

THO1: 9.3

TPOX: 8,10

vWA: 17

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC; HTB-174 ATCC; CRM-HTB-174 BCRC; 60455 
BCRJ; 0359 

供应限制

仅供科研使用

货号

ZQ0624

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

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