产品名称 |
HeLa-S3宫颈癌细胞 |
货号 |
ZQ0575 |
产品介绍 |
Hela S3细胞是由Puck·T·T、Marcus·P·I和Cieciura·S·J于1955年建系的。Hela S3细胞含HPV-18序列;角蛋白阳性。Hela S3细胞可用于与染色体突变、细胞营养、集落形成相关的哺乳动物细胞的克隆分析。 |
种属 |
人 |
性别/年龄 |
女/31岁 |
组织 |
子宫;颈部 |
疾病 |
腺癌,人乳头瘤病毒相关宫颈腺癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮 |
生长方式 |
贴壁 |
倍增时间 |
大约48小时 |
培养基和添加剂 |
MEM(含NEAA)培养基(中乔新舟 货号:ZQ-300)+10%FBS(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-2 |
STR位点信息 |
Amelogenin: X |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
|
基因表达 |
|
保藏机构 |
ATCC; CCL-2.2 ATCC; CRL-7924 DSMZ; ACC-161 ECACC; 87110901 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0575 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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Cross-sensitivity of methylating agents, hydroxyurea, and methotrexate in human tumor cells of the Mer- phenotype.
Cancer Res. 46:5009-5013(1986)
2、PubMed=3180844; DOI=10.1159/000132579
Chen T.-R.
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
Cytogenet. Cell Genet. 48:19-24(1988)
3、PubMed=1522048; DOI=10.1007/BF02634140
Kataoka E., Honma M., Ohnishi K., Sofuni T., Mizusawa H.
Application of highly polymorphic DNA markers to the identification of HeLa cell sublines.
In Vitro Cell. Dev. Biol. Anim. 28:553-556(1992)
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Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)
6、DOI=10.11418/jtca1981.17.3_101
Itami C., Ishida R., Utsumi K.R., Nakabayashi T.
Variation in three groups of HeLa cell sublines as revealed by karyotype analysis and O6-methylguaninedna-methyltransferase activity.
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7、PubMed=10338214; DOI=10.1016/S0092-8674(00)80760-6
Griffith J.D., Comeau L., Rosenfield S., Stansel R.M., Bianchi A., Moss H., de Lange T.
Mammalian telomeres end in a large duplex loop.
Cell 97:503-514(1999)
8、PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)
9、PubMed=14518029; DOI=10.1002/pros.10290
van Bokhoven A., Varella-Garcia M., Korch C.T., Johannes W.U., Smith E.E., Miller H.L., Nordeen S.K., Miller G.J., Lucia M.S.
Molecular characterization of human prostate carcinoma cell lines.
Prostate 57:205-225(2003)
10、PubMed=19450234; DOI=10.2144/000113089
Rahbari R., Sheahan T., Modes V., Collier P., Macfarlane C.M., Badge R.M.
A novel L1 retrotransposon marker for HeLa cell line identification.
BioTechniques 46:277-284(2009)
11、PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)
12、PubMed=23925245; DOI=10.1038/nature12064
Adey A.C., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R.-L., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)
13、PubMed=24185095; DOI=10.1038/nbt.2727
Burton J.N., Adey A.C., Patwardhan R.P., Qiu R.-L., Kitzman J.O., Shendure J.
Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions.
Nat. Biotechnol. 31:1119-1125(2013)
14、PubMed=26550473; DOI=10.1186/s13742-015-0091-4
Wu L., Zhang X.-L., Zhao Z.-K., Wang L., Li B., Li G.-B., Dean M., Yu Q.-C., Wang Y.-H., Lin X.-X., Rao W.-J., Mei Z.-L., Li Y., Jiang R.-Z., Yang H., Li F.-Q., Xie G.-Y., Xu L.-Q., Wu K., Zhang J., Chen J.-H., Wang T., Kristiansen K., Zhang X.-Q., Li Y.-R., Yang H.-M., Wang J., Hou Y., Xu X.
Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells.
GigaScience 4:51.1-51.17(2015)
15、PubMed=30175587; DOI=10.1021/acs.jproteome.8b00392
Robin T., Bairoch A., Muller M., Lisacek F., Lane L.
Large-scale reanalysis of publicly available HeLa cell proteomics data in the context of the Human Proteome Project.
J. Proteome Res. 17:4160-4170(2018)
16、PubMed=30778230; DOI=10.1038/s41587-019-0037-y
Liu Y.-S., Mi Y., Mueller T., Kreibich S., Williams E.G., Van Drogen A., Borel C., Frank M., Germain P.-L., Bludau I., Mehnert M., Seifert M., Emmenlauer M., Sorg I., Bezrukov F., Sloan-Bena F., Zhou H., Dehio C., Testa G., Saez-Rodriguez J., Antonarakis S.E., Hardt W.-D., Aebersold R.
Multi-omic measurements of heterogeneity in HeLa cells across laboratories.
Nat. Biotechnol. 37:314-322(2019)
17、PubMed=33205894; DOI=10.15252/msb.20209946
Schwabe D., Formichetti S., Junker J.P., Falcke M., Rajewsky N.
The transcriptome dynamics of single cells during the cell cycle.
Mol. Syst. Biol. 16:e9946.1-e9946.20(2020)
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