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Thp-1人单核细胞白血病(STR鉴定)
英文名:Thp-1
货号:ZQ0086
价格:¥1600.00
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Thp-1人单核细胞白血病(STR鉴定)

¥1600.00
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THP-1人单核细胞白血病专用培养基

¥550.00

配套完培,组合使用,省时省力!

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细胞套餐惊爆价

¥2150
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

Thp-1人单核细胞白血病

货号

ZQ0086

产品介绍

THP-1是一种人急性单核细胞白血病细胞系,最初从一位1岁患有急性单核细胞白血病的男孩的外周血中分离出来的单核细胞。该细胞对乳汁珠和激活的红细胞有吞噬作用,无表面和胞质免疫球蛋白。 可以用佛波醇 TPA诱导单核细胞分化。表达C3R和FcR;可作为转染宿主。也因其能够被诱导分化成单核/巨噬细胞,所以在免疫学研究中具有广泛的应用。例如:巨噬细胞分化与炎症模型、基因编辑研究、免疫调节作用研究、疾病模型构建与药物筛选、STING激动剂筛选模型、细胞凋亡和细胞周期研究、表观遗传调控研究等。这些应用展示了THP-1细胞系在免疫学研究中的多样性和重要性,为理解免疫调节和疾病机制提供了宝贵的工具。

【注意事项】:
1.该细胞为悬浮细胞。根据我司培养经验,建议传代时使用【半换液法】对细胞状态有利。当培养基颜色比较黄建议离心换液,培养基颜色变化不明显且细胞数量多可以进行补液,然后将细胞吹打用手指弹松后均匀移入两个新的 T25培养瓶中维续培养即可(1:2 传代)。
2. 该细胞在培养过程中,可能会有少量贴壁或聚集成小团生长,属于正常情况。传代时可以轻轻打散,如少许细胞已贴壁则传代时丢弃,小团生长的细胞正常传代培养即可。
3. 该细胞对细胞密度较为敏感,培养、传代时请注意保持细胞密度在合适的范围。
4.  该细胞较难复苏,复苏后需要约7天左右才可能恢复至正常状态。刚复苏后,细胞生长缓慢且会出现黑色细胞碎片,少量细胞碎片不影响细胞正常生长,待细胞恢复生长后用半换液法可以稀释碎片。
5. 细胞冻存时务请提高细胞密度,建议1个T25培养瓶中含有3-5×106细胞量时冻存一支。
6. 该细胞对血清质量较为敏感,我司建议您使用优质胎牛血清进行培养或选择订购我司配套THP-1完全培养液。

种属

性别/年龄

男/1岁

组织

外周血

疾病

急性单核细胞白血病

细胞类型

肿瘤细胞

形态学

单核细胞

生长方式

悬浮

倍增时间

大约26~70小时

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500+1%双抗(中乔新舟   货号:CSP006+β-Mer 终浓度:0.05mM(中乔新舟  货号:CSP005,例:500ml添加500ul

推荐完全培养基货号

ZM0086

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
TH01: 8,9.3
TPOX: 8,11
vWA: 16
D3S1358: 15,17
D21S11: 30,31.2
D18S51: 13,14
D8S1179: 10,14
FGA: 24,25
D19S433: 12.2,13
D2S1338: 17,18

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC; TIB-202 DSMZ; ACC-16 ECACC; 88081201

供应限制

仅供科研使用


货号

ZQ0086

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


论文标题: Exosomal ncRNAs profiling of mycobacterial infection identified miRNA-185-5p as a novel biomarker for tuberculosis
DOI: 10.1093/bib/bbab210
发表时间: 2021-11-05
期刊: BRIEFINGS IN BIOINFORMATICS
影响因子: 11.622
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://academic.oup.com/bib/article-abstract/22/6/bbab210/6309357


论文标题: 45S5 Bioglass® works synergistically with siRNA to downregulate the expression of matrix metalloproteinase-9 in diabetic wounds
DOI: 10.1016/j.actbio.2022.04.010
发表时间: 2022-04-12
期刊: Acta Biomaterialia
影响因子: 8.947
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S1742706122002148


论文标题: Calcium silicate enhances immunosuppressive function of MSCs to indirectly modulate the polarization of macrophages
DOI: 10.1093/rb/rbab056
发表时间: 2021-09-02
期刊: Regenerative Biomaterials
影响因子: 6.353
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://academic.oup.com/rb/article-abstract/8/6/rbab056/6408453


论文标题: Eltrombopag inhibits Type I interferon-mediated antiviral signaling by decreasing cellular iron
DOI: 10.1016/j.bcp.2021.114436
发表时间: 2021-02-01
期刊: BIOCHEMICAL PHARMACOLOGY
影响因子: 5.858
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0006295221000320


论文标题: The new mitochondrial uncoupler BAM15 induces ROS production for treatment of acute myeloid leukemia
DOI: 10.1016/j.bcp.2022.114948
发表时间: 2022-02-19
期刊: BIOCHEMICAL PHARMACOLOGY
影响因子: 5.858
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0006295222000429

论文标题: A novel role of VEPH1 in regulating AoSMC phenotypic switching
DOI: 10.1002/jcp.29736
发表时间: 2020-04-27
期刊: JOURNAL OF CELLULAR PHYSIOLOGY
影响因子: 5.546
货号: ZQ0086
产品名称: THP1 cells

原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29736


论文标题: AT1R Regulates Macrophage Polarization Through YAP and Regulates Aortic Dissection Incidence
DOI: 10.3389/fphys.2021.644903
发表时间: 2021-07-09
期刊: Frontiers in Physiology
影响因子: 4.566
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8299470/


论文标题: Knockdown of periostin attenuates 5/6 nephrectomy‐induced intrarenal renin–angiotensin system activation, fibrosis, and inflammation in rats
DOI: 10.1002/jcp.28849
发表时间: 2019-05-24
期刊: JOURNAL OF CELLULAR PHYSIOLOGY
影响因子: 4.522
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28849


论文标题: MiR-144-5p limits experimental abdominal aortic aneurysm formation by mitigating M1 macrophage-associated inflammation: Suppression of TLR2 and OLR1
DOI: 10.1016/j.yjmcc.2020.04.008
发表时间: 2020-04-09
期刊: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
影响因子: 4.133
货号: ZQ0086
产品名称: THP-1 monocytes
原文链接: https://www.sciencedirect.com/science/article/pii/S0022282820300900


论文标题: Supervillin Contributes to LPS-induced Inflammatory Response in THP-1 Cell-derived Macrophages
DOI: 10.1007/s10753-021-01551-7
发表时间: 2021-09-03
期刊: INFLAMMATION
影响因子: 4.092
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://link.springer.com/article/10.1007/s10753-021-01551-7


论文标题: mTORC1 signaling pathway regulates macrophages in choroidal neovascularization
DOI: 10.1016/j.molimm.2020.03.002
发表时间: 2020-03-12
期刊: MOLECULAR IMMUNOLOGY
影响因子: 3.641
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0161589019308636


论文标题: SIRT1-autophagy axis inhibits excess iron-induced ferroptosis of foam cells and subsequently increases IL-1Β and IL-18
DOI: 10.1016/j.bbrc.2021.05.011
发表时间: 2021-05-15
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 3.575
货号: ZQ0086
产品名称: THP-1 monocytes
原文链接: https://www.sciencedirect.com/science/article/pii/S0006291X21007816


论文标题: MicroRNA-216a Promotes Endothelial Inflammation by Smad7/IκBα Pathway in Atherosclerosis
DOI: 10.1155/2020/8864322
发表时间: 2020-11-18
期刊: DISEASE MARKERS
影响因子: 2.738
货号: ZQ0086
产品名称: THP-1 monocytes
原文链接: https://www.hindawi.com/journals/dm/2020/8864322/


论文标题: Comparative Analysis of Chemical Composition andAntibacterial and Anti-Inflammatory Activities of theEssential Oils from Chrysanthemum morifolium ofDifferent Flowering Stages and Different Parts
DOI: 10.1155/2022/5954963
发表时间: 2022-06-06
期刊: Evidence-based Complementary and Alternative Medicine
影响因子: 2.65
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.hindawi.com/journals/ecam/2022/5954963/


论文标题: Alteration of liver immunity by increasing inflammatory response during co-administration of methamphetamine and atazanavir
DOI: 10.1080/08923973.2020.1745829
发表时间: 2020-04-06
期刊: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
影响因子: 2.352
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/08923973.2020.1745829


论文标题: PCAT18, as a novel differentially regulated long noncoding RNA in adult acute myeloid leukemia patients revealed by next-generation sequencing
DOI: 10.1111/ijlh.13305
发表时间: 2020-10-02
期刊: International Journal of Laboratory Hematology
影响因子: 2.141
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1111/ijlh.13305


论文标题: Long non‑coding RNA MALAT1 regulates cholesterol accumulation in ox‑LDL‑induced macrophages via the microRNA‑17‑5p/ABCA1 axis
DOI: 10.3892/mmr.2020.10987
发表时间: 2020-04-01
期刊: Molecular Medicine Reports
影响因子: 2.1
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.spandidos-publications.com/10.3892/mmr.2020.10987


论文标题: Effect of HMGB1 on monocyte immune function in respiratory syncytial virus bronchiolitis
DOI: 10.3892/etm.2020.9507
发表时间: 2020-11-25
期刊: Experimental and Therapeutic Medicine
影响因子: 1.785
货号: ZQ0086
产品名称: THP-1 monocytes
原文链接: https://www.spandidos-publications.com/10.3892/etm.2020.9507


论文标题: Silencing p62 reduces ox-LDL-induced M1 polarization and inflammation in macrophages by inhibiting mTOR/NF-κB signaling pathways:
DOI: 10.1177/1721727X221110348
发表时间: 2022-07-16
期刊: European Journal of Inflammation
影响因子: 0.424
货号: ZQ0086
产品名称: THP-1 monocytes

原文链接: https://journals.sagepub.com/doi/abs/10.1177/1721727X221110348



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PubMed=16408098; DOI=10.1038/sj.leu.2404081
Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)


PubMed=19220422; DOI=10.1111/j.1600-0609.2009.01211.x
Kamihira S., Terada C., Sasaki D., Yanagihara K., Tsukasaki K., Hasegawa H., Yamada Y.
Aberrant p53 protein expression and function in a panel of hematopoietic cell lines with different p53 mutations.
Eur. J. Haematol. 82:301-307(2009)


PubMed=19377474; DOI=10.1038/ng.375; PMCID=PMC6711855
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FANTOM Consortium
Riken Omics Science Center
The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line.
Nat. Genet. 41:553-562(2009)


PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
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PubMed=22645513; DOI=10.3389/fendo.2011.00089; PMCID=PMC3355855
Keuper M., Dzyakanchuk A., Amrein K.E., Wabitsch M., Fischer-Posovszky P.
THP-1 macrophages and SGBS adipocytes -- a new human in vitro model system of inflamed adipose tissue.
Front. Endocrinol. 2:89.1-89.8(2011)


PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)


PubMed=22481983; DOI=10.2174/1874091X01206010016; PMCID=PMC3314867
Chauhan V., Howland M., Wilkins R.C.
Effects of alpha-particle radiation on microRNA responses in human cell-lines.
Open Biochem. J. 6:16-22(2012)


PubMed=23097634; DOI=10.1100/2012/205038; PMCID=PMC3477555
Chauhan V., Howland M.
Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.
ScientificWorldJournal 2012:205038.1-205038.9(2012)


PubMed=22674354; DOI=10.1002/ijc.27661
Battle R., Poole K., Haywood-Small S., Clark B., Woodroofe M.N.
Molecular characterisation of the monocytic cell line THP-1 demonstrates a discrepancy with the documented HLA type.
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