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CHO-K1仓鼠卵巢细胞亚株(种属鉴定)
英文名:CHO-K1
货号:ZQ0106
价格:¥1400.00
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CHO-K1仓鼠卵巢细胞亚株(种属鉴定)

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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

名称

CHO-K1仓鼠卵巢细胞亚株(种属鉴定)

货号

ZQ0106

产品介绍

CHO-K1是中国仓鼠卵巢细胞(Chinese Hamster Ovary,简称CHO细胞)源自成年中国仓鼠卵巢深入活体组织切片的CHO细胞的一个亚克隆,最初由T. T. Puck在1957年从一只成年中国仓鼠的卵巢组织中建立。这种细胞系因其易于转染、能够在生物制药行业用于生产重组蛋白和生物药物而被广泛使用。

种属

仓鼠

组织来源

卵巢  

形态学

成纤维细胞样

细胞类型

自发永生化细胞

倍增时间

~24 hours (DSMZ=ACC-110)

生长方式

贴壁

培养基和添加剂

90%F12K(中乔新舟  货号:ZQ-599+10%胎牛血清(中乔新舟  货号:ZQ500-A+1%双抗(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0106

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

保藏机构

AddexBio; P0017001/33
ATCC; CCL-61
ATCC; CRL-9618
BCRC; 60006
BCRJ; 0069
CCLV; CCLV-RIE 0134
CCLV; CCLV-RIE 0315
CCTCC; GDC0018
CLS; 603480
Coriell; GM15452
DSMZ; ACC-110
ECACC; 85051005
IBRC; C10136
ICLC; ATL98003
IZSLER; BS CL 15
JCRB; IFO50414
JCRB; JCRB9018
KCB; KCB 88028YJ
KCLB; 10061
NCBI_Iran; C645
RCB; RCB0285
RCB; RCB2330
RCB; RCB2869
TKG; TKG 0328
Ubigene; YC-C066

供应限制

仅供科研使用

货号

ZQ0106

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

论文标题: Transcriptional Expressions of ALDH1A1/B1 as Independent Indicators for the Survival of Thyroid Cancer Patients.
DOI: 10.3389/fonc.2022.821958
发表时间: 2022-02-23
期刊: Frontiers in Oncology
影响因子: 6.244
货号: ZQ0106
产品名称: CHO-K1 cells

原文链接: https://europepmc.org/article/pmc/pmc8905520?javascript_support=no

PubMed=10320750; DOI=10.1016/S0027-5107(99)00077-9
Hu T., Miller C.M., Ridder G.M., Aardema M.J.
Characterization of p53 in Chinese hamster cell lines CHO-K1, CHO-WBL, and CHL: implications for genotoxicity testing.
Mutat. Res. 426:51-62(1999)

PubMed=21804562; DOI=10.1038/nbt.1932
Xu X., Nagarajan H., Lewis N.E., Pan S.-K., Cai Z.-M., Liu X., Chen W.-B., Xie M., Wang W.-L., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W., Fan H.C., Wang J.-B., Gui Y.-T., Lee K.H., Betenbaugh M.J., Quake S.R., Famili I., Palsson B.O., Wang J.
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.
Nat. Biotechnol. 29:735-741(2011)

PubMed=21945585; DOI=10.1016/j.jbiotec.2011.09.014
Becker J., Hackl M., Rupp O., Jakobi T., Schneider J., Szczepanowski R., Bekel T., Borth N., Goesmann A., Grillari J., Kaltschmidt C., Noll T., Puhler A., Tauch A., Brinkrolf K.
Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing.
J. Biotechnol. 156:227-235(2011)

PubMed=22971049; DOI=10.1021/pr300476w
Baycin-Hizal D., Tabb D.L., Chaerkady R., Chen L., Lewis N.E., Nagarajan H., Sarkaria V., Kumar A., Wolozny D., Colao J., Jacobson E., Tian Y., O'Meally R.N., Krag S.S., Cole R.N., Palsson B.O., Zhang H., Betenbaugh M.J.
Proteomic analysis of Chinese hamster ovary cells.
J. Proteome Res. 11:5265-5276(2012)

DOI=10.3390/pr1030296
Wurm F.M.
CHO quasispecies -- implications for manufacturing processes.
Processes 1:296-311(2013)

PubMed=23873082; DOI=10.1038/nbt.2624
Lewis N.E., Liu X., Li Y.-X., Nagarajan H., Yerganian G., O'Brien E., Bordbar A., Roth A.M., Rosenbloom J., Bian C., Xie M., Chen W.-B., Li N., Baycin-Hizal D., Latif H., Forster J., Betenbaugh M.J., Famili I., Xu X., Wang J., Palsson B.O.
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.
Nat. Biotechnol. 31:759-765(2013)

PubMed=26993211; DOI=10.1016/j.jbiotec.2016.03.022
Klanert G., Jadhav V., Shanmukam V., Diendorfer A.B., Karbiener M., Scheideler M., Hernandez-Bort J.A., Grillari J., Hackl M., Borth N.
A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines.
J. Biotechnol. 235:150-161(2016)

DOI=10.3390/pr5020020
Wurm F.M., Wurm M.J.
Cloning of CHO cells, productivity and genetic stability -- a discussion.
Processes 5:20.1-20.13(2017)

PubMed=28544881; DOI=10.1016/j.cels.2017.04.009
Yusufi F.N.K., Lakshmanan M., Ho Y.-S., Loo B.L.-W., Ariyaratne P., Yang Y.-S., Ng S.K., Tan T.R.-M., Yeo H.C., Lim H.L., Ng S.W., Hiu A.-P., Chow C.P., Wan C., Chen S.-W., Teo G., Song G., Chin J.-X., Ruan X.-A., Sung K.W.-K., Hu W.-S., Yap M.G.-S., Bardor M., Nagarajan N., Lee D.-Y.
Mammalian systems biotechnology reveals global cellular adaptations in a recombinant CHO cell line.
Cell Syst. 4:530-542.e6(2017)

PubMed=32078082; DOI=10.1007/s10529-020-02841-0
Capella Roca B., Doolan P., Barron N., O'Neill F., Clynes M.
Altered gene expression in CHO cells following polyamine starvation.
Biotechnol. Lett. 42:927-936(2020)

PubMed=32619503; DOI=10.1016/j.ymben.2020.06.002
Szeliova D., Ruckerbauer D.E., Galleguillos S.N., Petersen L.B., Natter K., Hanscho M., Troyer C., Causon T., Schoeny H., Christensen H.B., Lee D.-Y., Lewis N.E., Koellensperger G., Hann S., Nielsen L.K., Borth N., Zanghellini J.
What CHO is made of: variations in the biomass composition of Chinese hamster ovary cell lines.
Metab. Eng. 61:288-300(2020)

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