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HBL-100人整合SV40基因的乳腺上皮细胞(STR鉴定)
英文名:HBL-100
货号:ZQ0370
价格:¥1000.00
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HBL-100人整合SV40基因的乳腺上皮细胞(STR鉴定)

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HBL-100人整合SV40基因的乳腺上皮细胞专用培养基

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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

HBL-100人整合SV40基因的乳腺上皮细胞

货号

ZQ0370

产品介绍

HBL-100是一株上皮细胞,E.V. Gaffney及其同事从一位没有乳癌家族史的供者乳汁中建立。 培养出来的细胞染色体组型在第7代时就不正常。电镜照片显示有微丝、张力原纤维和桥粒。 Southern转移表明有整合型SV40病毒基因,不可以当作正常细胞。

注意事项:
HBL-100细胞低密度时细胞状态要特别注意温度变化和和细胞震动,否则细胞易脱落,在细胞密度80%时细胞即可传代。建议使用Corning CellBIND(3289)培养瓶。

种属

性别/年龄

组织

整合SV40基因的乳腺上皮细胞

细胞类型

转化细胞系 

形态学

上皮

生长方式

贴壁

倍增时间

大约28~40小时 

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200+20%胎牛血清(中乔新舟 货号:ZQ0500+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0370

生物安全等级

BSL-1

STR位点信息

Amelogenin: X
D5S818:11,12
D13S317: 12
D7S820: 8,12
D16S539: 9,12
vWA: 16
TH01: 6,8
TPOX: 8
CSF1PO: 10
D19S433: 15
D21S11: 28,30
D18S51: 16 

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 

基因表达

 

保藏机构

ATCC; HTB-124 RCB; RCB0460

供应限制

仅供科研使用

货号

ZQ0370

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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