产品名称 |
HL-60人原髓细胞白血病细胞 |
货号 |
ZQ0433 |
产品介绍 |
HL-60是一株早幼粒细胞。 外周血白细胞来自一位患有急性粒-单核细胞白血病的36岁白人女性。 HL-60 自发分化,丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸可以促进分化。 细胞表现出吞噬活性,并对趋化刺激有响应。致癌基因myc表达阳性。 |
种属 |
人 |
性别/年龄 |
女/36岁 |
组织 |
外周血 |
疾病 |
急性早幼粒细胞白血病 |
细胞类型 |
肿瘤细胞 |
形态学 |
淋巴母细胞样 |
生长方式 |
悬浮 |
倍增时间 |
大约16~40小时 |
培养基和添加剂 |
IMDM(品牌:中乔新舟 货号:ZQ-900)+20%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
D5S818: 12 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
Complement, expressed; Fc, expressed |
基因表达 |
tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid; myc+ |
保藏机构 |
ATCC; CCL-240 BCRC; 60027 BCRJ; 0104 DSMZ; ACC-3 ECACC; 98070106 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0433 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
原文链接: https://www.frontiersin.org/articles/10.3389/fphar.2018.01045/full
2、PubMed=29491412; DOI=10.1038/s41388-018-0150-2
Gerlach D., Tontsch-Grunt U., Baum A., Popow J., Scharn D., Hofmann M.H., Engelhardt H., Kaya O., Beck J., Schweifer N., Gerstberger T., Zuber J., Savarese F., Kraut N.
The novel BET bromodomain inhibitor BI 894999 represses super-enhancer-associated transcription and synergizes with CDK9 inhibition in AML.
Oncogene 37:2687-2701(2018)
3、PubMed=30285677; DOI=10.1186/s12885-018-4840-5
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)
4、PubMed=30629668; DOI=10.1371/journal.pone.0210404
Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:E0210404-E0210404(2019)
5、PubMed=30670178; DOI=10.1016/j.jcf.2018.06.007
Jennings S., Ng H.P., Wang G.-S.
Establishment of a DeltaF508-CF promyelocytic cell line for cystic fibrosis research and drug screening.
J. Cyst. Fibros. 18:44-53(2019)
6、PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
7、PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
8、PubMed=31160637; DOI=10.1038/s41598-019-44491-x
Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K., Uphoff C.C., Drexler H.G.
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218-8218(2019)
9、PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
10、PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)
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K-562人慢性髓原白血病细胞 (STR鉴定) | ¥1350.00 | 购物车 》 |
支原体检测试剂盒 | ¥800.00 | 购物车 》 |
支原体清除试剂盒 | ¥600.00 | 购物车 》 |
慢病毒介导基因沉默或过表达 | ¥询价 | 购物车 》 |
荧光素酶标记 | ¥询价 | 购物车 》 |
绿/红色荧光蛋白标记 | ¥询价 | 购物车 》 |
胎牛血清 | ¥7328.00 | 购物车 》 |
支原体抑制剂 10 mg/ml; 1 ml(定制) | ¥1808.00 | 购物车 》 |
IMDM 基础培养基 | ¥96.00 | 购物车 》 |
IMDM 完全培养基 | ¥650.00 | 购物车 》 |
人源细胞STR鉴定服务 | ¥900.00 | 购物车 》 |