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人皮炎、哮喘定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Dermatitis and Asthma qPCR Array Kit (GQH-DER) is designed to facilitate gene expression profiling of 88 key genes involved in the pathogenesis of atopic dermatitis (or eczema) and asthma, which is frequently associated with this chronic inflammatory skin disorder. Genes are also included for related conditions such as allergic contact dermatitis, seborrheic dermatitis, ichthyosis, and eczema herpeticum. Brief examples of how included genes may be grouped according to pathology are shown below:
Atopic dermatitis: CCL11, IFNG, IL13, IL4, SOCS3, FLG
Asthma: HNMT, ADRB2, ALOX5, PLA2G7, PHF11
Allergic contact dermatitis: IL10, IL17A, TNF, CD4, CXCR3
Seborrheic dermatitis: IDH2, SETBP1, ZNF750, ALOX12B, BTD
Ichthyosis: LOR, ABC12, KRT1, KRT14, STS
Eczema herpeticum: TSLP, IRF2, IRF7, IL7R, KLK12
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Dermatitis and Asthma qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABCA12 |
CCL11 |
CGA |
EPX |
HNMT |
IL18 |
IL9 |
LEP |
PAFAH1B1 |
SCGB3A2 |
TAC1 |
β-actin |
|
B |
ADA |
CCL17 |
COL6A5 |
FAF2 |
HRH1 |
IL2 |
IRAK3 |
LOR |
PDE4A |
SELE |
TBX21 |
GAPDH |
|
C |
ADRB2 |
CCL18 |
CSF2 |
FCER1A |
ICAM1 |
IL3 |
IRF2 |
LTC4S |
PHF11 |
SETBP1 |
TGM1 |
LDHA |
|
D |
ALOX12B |
CCL22 |
CXCR3 |
FCER1G |
IDH2 |
IL31RA |
IRF7 |
MS4A2 |
PHYH |
SLCO2A1 |
TMEM79 |
NONO |
|
E |
ALOX5 |
CCL27 |
CYSLTR1 |
FCER2 |
IFNG |
IL4 |
KLK12 |
NACA |
PLA2G7 |
SOCS3 |
TNF |
PPIH |
|
F |
ALOX5AP |
CCL5 |
CYSLTR2 |
FLG |
IL10 |
IL4R |
KLK7 |
NAT2 |
PTGDR |
SPINK5 |
TSLP |
GDC |
|
G |
BCL7B |
CD4 |
DPP10 |
HAVCR1 |
IL13 |
IL5 |
KRT1 |
NR3C1 |
PTGDR2 |
STAT3 |
VCAM1 |
PPC |
|
H |
BTD |
CD40LG |
DSG1 |
HLA-G |
IL17A |
IL7R |
KRT14 |
ORMDL3 |
RNASE3 |
STS |
ZNF750 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK048 |
|
产地 |
美国 |
|
缩写 |
GQH-DER |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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