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人I型糖尿病(青少年，胰岛素依赖型)定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Diabetes, Type I (Juvenile, Insulin-dependent) qPCR Array Kit (GQH-DB1) is designed to facilitate gene expression profiling of 88 key genes involved in the development and progression of insulin-dependent diabetes mellitus. Type 1 diabetes mellitus is characterized by abnormally high blood sugar levels resultant of a disorder in glucose homeostasis. Brief examples of how included genes may be grouped according to function are shown below:
Blood glucose regulation: INS, INSR, SLC2A2, SLC30A8, ABCC8
Autoimmunity: GAD1, GAD2, LTA, HLA-A, OAS1
Fat metabolism: ADIPOQ, LPL, PON1, LEP, RETN
Cholesterol regulation: APOA1, APOB, LPA, CETP, AMBP
Hormone regulation: PREP, IGF1, SST, HGF, REN
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Diabetes, Type I (Juvenile, Insulin-dependent) qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABCC8 |
AKR1B1 |
CETP |
GAD1 |
HLA-A |
IAPP |
IGFBP3 |
IRS1 |
NOS3 |
PTPRN |
SLC2A4 |
β-actin |
|
B |
ACE |
ALB |
CLEC16A |
GAD2 |
HLA-DQA1 |
ICA1 |
IL10 |
ITPR3 |
OAS1 |
PTPRN2 |
SLC30A8 |
GAPDH |
|
C |
ADIPOQ |
AMBP |
COG2 |
GCG |
HLA-DQB1 |
IFIH1 |
IL1B |
KCNJ11 |
PAX4 |
REN |
SST |
LDHA |
|
D |
ADRB3 |
AOC3 |
CPE |
GCK |
HLA-DRB1 |
IFNG |
IL2RA |
LEP |
PDX1 |
RETN |
SUMO4 |
NONO |
|
E |
AGER |
APOA1 |
CRP |
GH1 |
HNF1A |
IGF1 |
IL4 |
LPA |
PON1 |
SERPINE1 |
TG |
PPIH |
|
F |
AGT |
APOB |
CTLA4 |
GHR |
HNF1B |
IGF2 |
IL6 |
LPL |
PPARG |
SH2D1A |
TPO |
GDC |
|
G |
AGTR1 |
CCR5 |
FOXP3 |
HBA2 |
HNF4A |
IGFBP1 |
INS |
LTA |
PREP |
SLC19A2 |
VDR |
PPC |
|
H |
AIRE |
CD79A |
G6PC2 |
HGF |
HSPD1 |
IGFBP2 |
INSR |
NEUROD1 |
PTPN3 |
SLC2A2 |
WFS1 |
NT |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK050 |
|
产地 |
美国 |
|
缩写 |
GQH-DB1 |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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