产品名称 |
WEHI-3小鼠白血病细胞 |
货号 |
ZQ0848 |
产品介绍 |
该细胞表达补体C3受体(C3R),产生溶菌酶、粒细胞集落刺激活性(CSA)、IL-3。4ng/mlLPS可以抑制WEHI-3的生长,更高浓度可以阻断其生长;30~40μg/ml右旋葡聚糖也可抑制其生长;可以吞噬乳胶颗粒,但无毒性;可吞噬酵母聚糖和BCG,并阻断该细胞的生长;该细胞仅表现出微弱的抗体依赖的细胞介导的细胞毒反应。鼠痘病毒阴性。 |
种属 |
小鼠 |
组织来源 |
外周血 |
形态学 |
巨噬细胞样 |
细胞类型 |
肿瘤细胞 |
倍增时间 |
18 hours (Note=In 2% FCS) (PubMed=3005406). |
生长方式 |
半贴半悬 |
培养基和添加剂 |
IMDM(中乔新舟 货号:ZQ-900)+10%胎牛血清(中乔新舟 货号:ZQ0500)+0.05 mM 2-mercaptoethanol(中乔新舟 货号:CSP005)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; TIB-68 BCRC; 60176 CLS; 400381 JCRB; JCRB9093 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0848 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=5259325; DOI=10.1093/jnci/43.4.963
Warner N.L., Moore M.A.S., Metcalf D.
A transplantable myelomonocytic leukemia in BALB-c mice: cytology, karyotype, and muramidase content.
J. Natl. Cancer Inst. 43:963-982(1969)
PubMed=4543620; DOI=10.3181/00379727-144-37688
Rollinghoff M., Warner N.L.
Specificity of in vivo tumor rejection assessed by mixing immune spleen cells with target and unrelated tumor cells.
Proc. Soc. Exp. Biol. Med. 144:813-818(1973)
PubMed=6170720; DOI=10.1084/jem.154.5.1419
McKean D.J., Infante A.J., Nilson A., Kimoto M., Fathman C.G., Walker E., Warner N.L.
Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells.
J. Exp. Med. 154:1419-1431(1981)
PubMed=6687612
de Weger R.A., van Loveren H., van Basten C.D.H., Oskam R., van der Zeijst B.A.M., Den Otter W.
Functional activities of the NCTC 1469 macrophage-like cell line: comparison of the NCTC 1469 cell line with various other macrophage-like cell lines.
J. Reticuloendothel. Soc. 33:55-66(1983)
PubMed=3005406; DOI=10.4049/jimmunol.136.6.2181
Morel P.A., Manolagas S.C., Provvedini D.M., Wegmann D.R., Chiller J.M.
Interferon-gamma-induced IA expression in WEHI-3 cells is enhanced by the presence of 1,25-dihydroxyvitamin D3.
J. Immunol. 136:2181-2186(1986)
PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x
Leenen P.J.M., Jansen A.M.A.C., van Ewijk W.
Murine macrophage cell lines can be ordered in a linear differentiation sequence.
Differentiation 32:157-164(1986)
PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)
PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)
产品名称 | 价格 | 指令 |
HL-60人原髓细胞白血病细胞 (STR鉴定) | ¥1600.00 | 购物车 》 |
Raw264.7小鼠单核巨噬细胞白血病细胞(种属鉴定)[套餐促销] | ¥1350.00 | 购物车 》 |
RBL-2H3大鼠嗜碱性细胞白血病细胞(种属鉴定) | ¥1350.00 | 购物车 》 |
TF-1人血液白血病细胞 (STR鉴定) | ¥1500.00 | 购物车 》 |
RBL-1大鼠嗜碱性粒细胞性白血病细胞(种属鉴定) | ¥1200.00 | 购物车 》 |
L1210小鼠白血病细胞(种属鉴定) | ¥1200.00 | 购物车 》 |
HuT 78人T淋巴细胞白血病细胞 (STR鉴定) | ¥1500.00 | 购物车 》 |
HEL人红白细胞白血病细胞 (STR鉴定) | ¥1350.00 | 购物车 》 |