产品名称 |
SW480人结肠癌细胞 |
货号 |
ZQ0063 |
产品介绍 |
SW480是一种人结肠腺癌细胞系,SW480和SW620均源自同一位男性患者,其中SW480是原发性结肠腺癌。最初是从一位50岁白人男性患者的直肠腺癌组织中分离得到的。这个细胞系在癌症研究中非常有用,尤其是在研究结直肠癌的生物学行为和药物反应方面。该细胞的CSAp和结肠抗原3阴性,角蛋白免疫过氧化物酶染色阳性。该细胞的p53基因第273位密码子的G->A突变导致Arg->His替代,第309位密码子的C->T突变导致Pro->Ser替代。该细胞p53蛋白表达水平升高,癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性,但未检测到癌基因N-myc的表达。此外,该细胞不表达细胞基质裂解酶Matrilysin(一种与肿瘤入侵相关的金属蛋白酶)。有报道称该细胞表达GM-CSF。此外,该细胞K-RAS蛋白第12位氨基酸Gly突变为Val(c.35G>T),常被用于PCR检测该位点突变的阳性对照。该细胞是研究结直肠癌的重要工具,为科学家们提供了一个模型系统,用于研究疾病的进展和开发新的治疗方法。
注意事项: |
种属 |
人 |
性别/年龄 |
男/50岁 |
组织 |
结肠 |
疾病 |
杜克斯氏B型,结肠直肠腺癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮 |
生长方式 |
贴壁 |
倍增时间 |
大约24~50小时 |
培养基和添加剂(默认) |
L-15基础(中乔新舟 货号:ZQ-1100)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) 注意默认方案培养条件:100%空气;37℃ |
培养方案B(可选) |
DMEM (中乔新舟 货号:ZQ-100)+10%FBS(中乔新舟 货号:ZQ0500)+1%双抗(中乔新舟 货号:CSP006) 注意B方案培养条件:95%空气,5%CO2;37℃ |
推荐默认完全培养基货号 |
|
B方案完全培养基货号 |
|
生物安全等级 |
BSL-1 |
默认方案培养条件 |
100%空气;37℃ |
STR位点信息 |
Amelogenin: X |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC;DSMZ CCL-228;ECACC ACC-313;87092801 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0063 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1毫升冻存管*1支(细胞量约为5个10^5细胞/瓶)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
论文标题microRNA-96通过调节AMPKα2-FTO-m6A/MYC轴促进结直肠癌的发生和发展
DOI: 10.1186/s13046-020-01731-7
发表时间: 2020-11-12
期刊:实验和临床癌症研究杂志
影响因子: 7.068
货号:ZQ0063
产品名称:SW480细胞
原文链接:https://link . springer . com/article/10.1186/s 13046-020-01731-7
论文标题: Knockdown of SFRS9 Inhibits Progression of Colorectal Cancer Through Triggering Ferroptosis Mediated by GPX4 Reduction
DOI: 10.3389/fonc.2021.683589
发表时间: 2021-07-16
期刊: Frontiers in Oncology
影响因子: 6.244
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8322952/
论文标题: Integrating network pharmacology deciphers the action mechanism of Zuojin capsule in suppressing colorectal cancer
DOI: 10.1016/j.phymed.2021.153881
发表时间: 2021-12-09
期刊: PHYTOMEDICINE
影响因子: 5.34
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0944711321004219
论文标题: Kinase GSK3β functions as a suppressor in colorectal carcinoma through the FTO-mediated MZF1/c-Myc axis
DOI: 10.1111/jcmm.16291
发表时间: 2021-02-02
期刊: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子: 5.31
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16291
论文标题: The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism
DOI: 10.1007/s00432-020-03179-w
发表时间: 2020-03-09
期刊: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
影响因子: 3.656
货号: ZQ0063
产品名称: SW-480 cells
原文链接: https://link.springer.com/article/10.1007/s00432-020-03179-w 论文标题: MiR-346-5p promotes colorectal cancer cell proliferation in vitro and in vivo by targeting FBXL2 and activating the β-catenin signaling pathway
DOI: 10.1016/j.lfs.2020.117300
发表时间: 2020-01-14
期刊: LIFE SCIENCES
影响因子: 3.647
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320520300473
论文标题: Sulforaphane suppresses carcinogenesis of colorectal cancer through the ERK/Nrf2‑UDP glucuronosyltransferase 1A metabolic axis activation
DOI: 10.3892/or.2020.7495
发表时间: 2020-02-10
期刊: ONCOLOGY REPORTS
影响因子: 3.417
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.spandidos-publications.com/10.3892/or.2020.7495
论文标题: Brusatol Inhibits Proliferation and Metastasis of Colorectal Cancer by Targeting and Reversing the RhoA/ROCK1 Pathway
DOI: 10.1155/2022/7132159
发表时间: 2022-05-18
期刊: Biomed Research International
影响因子: 3.411
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.hindawi.com/journals/bmri/2022/7132159/
论文标题: LINC01198 promotes colorectal cancer cell proliferation and inhibits apoptosis via Notch signaling pathway.
DOI: 10.26355/eurrev_202008_22641
发表时间: 2020-08-01
期刊: European Review for Medical and Pharmacological Sciences
影响因子: 3.024
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://europepmc.org/article/med/32894550?javascript_support=no
论文标题: miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα
DOI: 10.1186/s12885-018-5245-1
发表时间: 2019-01-08
期刊: BMC CANCER
影响因子: 2.933
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://link.springer.com/article/10.1186/s12885-018-5245-1
论文标题: Evodiamine inhibits vasculogenic mimicry in HCT116 cells by suppressing hypoxia-inducible factor 1-alpha-mediated angiogenesis
DOI: 10.1097/CAD.0000000000001030
发表时间: 2021-01-08
期刊: ANTI-CANCER DRUGS
影响因子: 2.248
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861498/
论文标题: Piperine inhibits colorectal cancer migration and invasion by regulating STAT3/Snail-mediated epithelial–mesenchymal transition
DOI: 10.1007/s10529-020-02923-z
发表时间: 2020-06-04
期刊: BIOTECHNOLOGY LETTERS
影响因子: 1.977
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://link.springer.com/content/pdf/10.1007/s10529-020-02923-z.pdf
论文标题: Two new compounds from Carpesium abrotanoides
DOI: 10.1016/j.phytol.2020.08.012
发表时间: 2020-09-17
期刊: Phytochemistry Letters
影响因子: 1.459
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S187439002030639X
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Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
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