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COLO 205人结肠癌细胞(STR鉴定)
英文名:COLO 205
货号:ZQ0124
价格:¥1350.00
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COLO 205人结肠癌细胞(STR鉴定)

¥1350.00
+

COLO 205人结肠癌细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥1700 ¥1980.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

COLO 205人结肠癌细胞

货号

ZQ0124

产品介绍

COLO 205是一种人结肠癌细胞系,最初由T·U·Sample等人于1957年从一位70岁白人男性结肠癌患者的腹水中分离得到。在取出液体标本之前,患者已经用5-氟尿嘧啶治疗了4-6周。这些细胞具有上皮细胞形态,广泛用于结直肠癌相关的生物医学研究,尤其是在癌症生物学、药物反应和转移机制的研究领域。

COLO 205细胞具有超三倍体核型,并且在异种移植到免疫缺陷小鼠中时,能够形成高分化腺癌。这些细胞表达多种与致癌和肿瘤抑制途径相关的关键蛋白,使其成为药理学实验和癌症研究的宝贵模型。它们对肿瘤坏死因子相关凋亡诱导配体(TRAIL)的反应性,使它们适合用于凋亡研究。

此外,COLO 205细胞已被广泛用于研究多种化疗药物的药效学,为理解结直肠癌治疗中的药物作用机制和耐药性提供了重要见解。利用COLO 205细胞系的研究显著推进了我们对结直肠腺癌典型生物学行为的理解,包括细胞增殖、分化以及与抗癌药物的相互作用。

注意事项:
COLO 205细胞低密度形态呈圆形,高密度时形态呈大部分悬浮细胞为圆形和少量贴壁细胞为圆形和小纺锤形。80%的细胞可以直接冲下来,余下的细胞可以选择消化收集。

种属

性别/年龄

男性/70岁

组织

结肠;来源于转移部位:腹水

疾病

杜克斯氏D型,结肠直肠腺癌

细胞类型

肿瘤细胞

形态学

上皮

生长方式

混合、贴壁和悬浮

倍增时间

大约20~26.76小时 

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200+10%FBS(品牌:中乔新舟 货号:ZQ500-A+1%双抗(中乔新舟   货号:CSP006

推荐完全培养基货号

ZM0124

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

D3S1358: 16
TH01: 8,9
D21S11: 30.2,33.2
D18S51: 18
Penta_E: 13,15
D5S818: 10,13
D13S317: 10,12
D7S820: 9,10
D16S539: 12,13
CSF1PO: 11,12
Penta_D: 9,11
vWA: 15
D8S1179: 9,14
TPOX: 11
FGA: 21,23
D19S433: 13,14
D2S1338: 17,18

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

***

基因表达

*** 

保藏机构

ATCC; CCL-222 BCRC; 60054 ECACC; 87061208

供应限制

仅供科研使用

货号

ZQ0124

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


1、论文标题: Knockdown of SFRS9 Inhibits Progression of Colorectal Cancer Through Triggering Ferroptosis Mediated by GPX4 Reduction
DOI: 10.3389/fonc.2021.683589
发表时间: 2021-07-16
期刊: Frontiers in Oncology
影响因子: 6.244
货号: ZQ0124
产品名称: Colo205 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8322952/


2、论文标题: miR-524-5p inhibits angiogenesis through targeting WNK1 in colon cancer cells
DOI: 10.1152/ajpgi.00369.2019
发表时间: 2020-04-07
期刊: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
影响因子: 3.725
货号: ZQ0124
产品名称: COLO205  cells

原文链接: https://journals.physiology.org/doi/abs/10.1152/ajpgi.00369.2019



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9、PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457
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10、PubMed=26589293; DOI=10.1186/s13073-015-0240-5
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11、PubMed=26537799; DOI=10.1074/mcp.M115.051235
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13、PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
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