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Kasumi-1人红白血病细胞 (STR鉴定)(暂不提供)
英文名:Kasumi-1
货号:ZQ0426
价格:¥1800.00
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推荐组合

Kasumi-1人红白血病细胞 (STR鉴定)(暂不提供)

¥1800.00
+

Kasumi-1人红白血病细胞专用培养基

¥700.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥2500
加入购物车
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

Kasumi-1人红白血病细胞

货号

ZQ0426

产品介绍

该细胞系是从一名急性髓系白血病(AML)患者的外周血中建立的。这是一个8号21号染色体易位的白血病细胞系。这种易位将AML1与ETO(或MTG8)基因并列,产生融合基因AML1-ETO(也称为AML1-mtg或RUNX1-CBF2T1)。因此,细胞产生嵌合的AML1-ETO蛋白。该蛋白下调CEBPA mRNA、蛋白和DNA结合活性,这对粒细胞的分化至关重要。在增殖实验中,培养的细胞对白细胞介素-3 (IL-3)、白细胞介素-6、粒细胞集落刺激因子(G-CSF)、粒细胞顶体噬菌体CSF (GM-CSF)有反应,但对IL-1和IL-5无反应。该细胞复苏后需要两周左右恢复正常生长。

Kasumi-1细胞具有髓细胞和巨噬细胞谱系的特征,使其在髓细胞分化的研究中特别有用。这些细胞在与TPA (phorbol 12-肉豆蔻酸13-乙酸酯)一起培养时可以被诱导分化为巨噬细胞样细胞,为探索髓系谱系承诺和分化的途径提供了一个强大的系统。这种分化能力增强了Kasumi-1细胞在AML生物学和更广泛的髓细胞发育过程研究中的效用。

注意事项:
a) 该细胞复苏后成活率较低,会出现大量死细胞和死细胞碎片,培养两周后有所好转。建议每1-2周对细胞进行1000rpm,离心5 min,弃掉上清,加入新鲜完全培养液,可以去掉部分细胞碎片和颗粒。培养过程中会出现死细胞和细胞碎片,收到邮寄的活细胞的用户若发现培养物内有部分死细胞和细胞碎片,此为正常现象。
b) 细胞培养过程中会有轻微聚团,轻轻吹打开即可。当细胞密度较大或者培养液变黄时,需要及时进行半换液或者完全换液。
c) 该细胞对血清质量较为敏感,建议您使用优质血清进行培养。
d) 请注意保持细胞密度在合适的范围(3x105 ~ 3x106 /ml),不能过稀。

种属

性别/年龄

男/7岁

组织

外周血

疾病

急性成髓细胞白血病

细胞类型

成髓细胞

形态学

成髓细胞

生长方式

悬浮

倍增时间

  ~40-72小时

培养基和添加剂

RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟  货号:CSP006)+1% Sodium Pyruvate 100 mM Solution(中乔新舟  货号:CSP003) 1%L-alanyl-L-glutamine(中乔新舟   货号:CSP004

推荐完全培养基货号

ZM0426

生物安全等级

BSL-1

STR位点信息

D5S818: 9,11
D13S317: 11,13
D7S820: 8,11
D16S539: 9,12
vWA: 14
THO1: 6,9
Amelogenin: X
TPOX: 8,9
CSF1PO: 10,12

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

  ***

基因表达

  ***

保藏机构

ATCC; CRL-2724 DSMZ; ACC-220

供应限制

仅供科研使用

货号

ZQ0426

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
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Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

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