产品名称 |
Kasumi-1人红白血病细胞 |
货号 |
ZQ0426 |
产品介绍 |
该细胞系是从一名急性髓系白血病(AML)患者的外周血中建立的。这是一个8号21号染色体易位的白血病细胞系。这种易位将AML1与ETO(或MTG8)基因并列,产生融合基因AML1-ETO(也称为AML1-mtg或RUNX1-CBF2T1)。因此,细胞产生嵌合的AML1-ETO蛋白。该蛋白下调CEBPA mRNA、蛋白和DNA结合活性,这对粒细胞的分化至关重要。在增殖实验中,培养的细胞对白细胞介素-3 (IL-3)、白细胞介素-6、粒细胞集落刺激因子(G-CSF)、粒细胞顶体噬菌体CSF (GM-CSF)有反应,但对IL-1和IL-5无反应。该细胞复苏后需要两周左右恢复正常生长。
Kasumi-1细胞具有髓细胞和巨噬细胞谱系的特征,使其在髓细胞分化的研究中特别有用。这些细胞在与TPA (phorbol 12-肉豆蔻酸13-乙酸酯)一起培养时可以被诱导分化为巨噬细胞样细胞,为探索髓系谱系承诺和分化的途径提供了一个强大的系统。这种分化能力增强了Kasumi-1细胞在AML生物学和更广泛的髓细胞发育过程研究中的效用。
注意事项: |
种属 |
人 |
性别/年龄 |
男/7岁 |
组织 |
外周血 |
疾病 |
急性成髓细胞白血病 |
细胞类型 |
成髓细胞 |
形态学 |
成髓细胞 |
生长方式 |
悬浮 |
倍增时间 |
~40-72小时 |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006)+1% Sodium Pyruvate 100 mM Solution(中乔新舟 货号:CSP003) 1%L-alanyl-L-glutamine(中乔新舟 货号:CSP004) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
D5S818: 9,11 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-2724 DSMZ; ACC-220 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0426 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)
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