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MDA-MB-415人乳腺腺癌细胞(STR鉴定)
英文名:MDA-MB-415
货号:ZQ0354
价格:¥1350.00
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推荐组合

MDA-MB-415人乳腺腺癌细胞(STR鉴定)

¥1350.00
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MDA-MB-415人乳腺腺癌细胞专用培养基

¥350.00 ¥480.00

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细胞套餐惊爆价

¥1700
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

MDA-MB-415人乳腺腺癌细胞

货号

ZQ0354

产品介绍

这株细胞表达WNT7B癌基因。 8168088]. 带瘤患者来自巴拉圭,虽然填报的是白人,但细胞表型存在G6PD A型,显示其属于混血。细胞株形成平展延伸的上皮细胞样,在电镜下呈现结节,伴随着延伸的微管和微板。不容易用胰酶消化。

注意事项:
1)MDA-MB-415细胞呈块状生长,细胞生长不会100%融合。
2)消化时若细胞大部分变小细胞团和圆细胞并脱落即可终止消化。细胞消化后传代培养需48小时后换液。
3)细胞冻存后复苏生长慢,每两天换液一次,需要一周左右可传代。

种属

性别/年龄

/38岁

组织

乳腺/乳房;来源于转移部位:胸腔积液

疾病

腺癌

细胞类型

 肿瘤细胞

形态学

上皮的

生长方式

贴壁

倍增时间

大约88.87小时

培养基和添加剂(默认)

DMEM (中乔新舟  货号:ZQ-100)+10%FBS(中乔新舟 货号:ZQ0500+1%双抗(中乔新舟   货号:CSP006

注意默认方案培养条件:95%空气,5%CO2;37℃

培养方案B(可选)

L-15(中乔新舟  货号:ZQ-1100+15%FBS(中乔新舟  货号:ZQ0500+10ug/ml胰岛素(中乔新舟  货号CSP001-10+10ug/ml谷胱甘肽+1%P/S(中乔新  货号:CSP006

注意B方案培养条件:100%空气;37℃

推荐默认完全培养基货号

ZM0354

B方案完全培养基货号

ZM0354-B

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

CSF1PO: 10,12

D13S317: 11,13

D16S539: 13

D5S818: 11,13

D7S820: 9,10

TH01: 7,9.3

TPOX: 8

vWA: 17

默认方案培养条件

95%空气,5%CO2;37℃

抗原表达/受体表达

***

基因表达

***

保藏机构

ATCC; HTB-128

供应限制

仅供科研使用

货号

ZQ0354

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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